Rhodiola rosea in vitro culture - phytochemical analysis and antioxidant action

Abstract

Various Rhodiola rosea organs and tissues from in vitro culture were studied in two areas: searching for the biological active four phenolic compounds and measurement of antioxidant activity of dry residues of EtOH-extracts soluble in PBS using chemiluminescence method. For phytochemical investigation HPLC method was used. Salidroside was typical for organs of intact plant, rosavin for roots of different origin and shoots of intact plants, triandrin was more typical for tissue cultured in vitro, but it was found in all studied samples. Caffeic acid was detected mainly in green and yellow lines of callus, less in other Rhodiola rosea plant material. The highest antioxidant activity showed the extract from 1,5-years-old callus of green and yellow line, which contained caffeic acid and triandrin. Further observations are in progress.

Keywords

• Rhodiola rosea
• Crassulaceae
• tissue culture
• phenolics
• antioxidant activity
• Luminol-dependent chemiluminescence