Anti-inflammatory effect of trans-anethol in a rat model of myocardial ischemia-reperfusion injury

Marwa Matboli; Amany Helmy Hasanin; Shaimaa Hamady; Eman Khairy; Reham Hussein Mohamed; Yasmin M. Aboul-Ela; Mona Hussien Raafat; Sara Abdel Gawad Elsebay; Hossam Y. Emam; Rania Shamekh Shamekh; Sara H.A. Agwa

Biomedicine & Pharmacotherapy (Jun 2022)

Anti-inflammatory effect of trans-anethol in a rat model of myocardial ischemia-reperfusion injury

Marwa Matboli,
Amany Helmy Hasanin,
Shaimaa Hamady,
Eman Khairy,
Reham Hussein Mohamed,
Yasmin M. Aboul-Ela,
Mona Hussien Raafat,
Sara Abdel Gawad Elsebay,
Hossam Y. Emam,
Rania Shamekh Shamekh,
Sara H.A. Agwa

Affiliations

Marwa Matboli: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Corresponding authors.
Amany Helmy Hasanin: Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Shaimaa Hamady: Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt
Eman Khairy: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Reham Hussein Mohamed: Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Yasmin M. Aboul-Ela: Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Mona Hussien Raafat: Histology and Cell Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Sara Abdel Gawad Elsebay: Histology and Cell Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Hossam Y. Emam: Anatomy Department, Faculty of Medicine, Cairo University, Egypt
Rania Shamekh Shamekh: Department of Pathology, University of South Florida, Tampa, FL 33620, USA
Sara H.A. Agwa: Clinical Pathology and Molecular Genomics Unit, Faculty of Medicine, Medical Ain Shams Research Institute (MASRI), Ain Shams University, Cairo, Egypt; Corresponding authors.

Journal volume & issue: Vol. 150
p. 113070

Abstract

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Myocardial ischemia‑reperfusion injury (MI/R) is considered a main risk factor for global cardiac mortality and morbidity, for which no effective treatment exists. Both inflammation and epigenetic regulation play a pivotal role in the early stage of MI/R. The present study aimed at investigating the prospective anti-inflammatory role of trans-anethole (TNA) in targeting MI/R and its related mechanism in upregulating the expression of the inflammatory and cardiac-related gene (VAV3), and its epigenetic regulators (lncRNA-JRKL-AS1 and miR-1298) that were retrieved from in-silico data analysis in an ischemia/reperfusion (I/R) rat model. Materials & methods: TNA was administered in 3 doses (50, 100, and 200 mg/kg), 15 min prior to coronary ligation in male Wistar rats. The left ventricular end-diastolic pressure and dP/dtmax were assessed. Histopathological, biochemical, and molecular analyses were performed to assess the effects of TNA pre-treatment on the I/R rats model. Results: TNA alleviated the I/R-induced cardiac injury pathologically and improved the cardiac function tests and enzymes. At the molecular level, TNA upregulated the expression level of the retrieved RNA-based panel (VAV3 mRNA/miR-1298/lncRNA JRKL-AS1). At the protein level, TNA decreased the cardiac content of the pro-inflammatory cytokine TNF-α. Conclusion: TNA has demonstrated a potential ability to alleviate the cardiac injury and attenuate the inflammatory response following ischemia-reperfusion in the rat model through modulation of the expression of RNA panel (VAV3 mRNA/miR-1298/lncRNA JRKL-AS1) and TNF- α protein.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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