Generation of SST-P2A-mCherry reporter human embryonic stem cell line using the CRISPR/Cas9 system (WAe001-A-2C)

Tongran Zhang; Feng Zhang; Nannan Wang; Tao Xu; Lingqiang Zhu; Lihua Chen; Huisheng Liu

Stem Cell Research (Jun 2024)

Generation of SST-P2A-mCherry reporter human embryonic stem cell line using the CRISPR/Cas9 system (WAe001-A-2C)

Tongran Zhang,
Feng Zhang,
Nannan Wang,
Tao Xu,
Lingqiang Zhu,
Lihua Chen,
Huisheng Liu

Affiliations

Tongran Zhang: Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China; Guangzhou Laboratory, Guangzhou 510006, China
Feng Zhang: Guangzhou Laboratory, Guangzhou 510006, China; The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510006, China
Nannan Wang: Guangzhou Laboratory, Guangzhou 510006, China; College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
Tao Xu: School of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510180, China; Guangzhou Laboratory, Guangzhou 510006, China
Lingqiang Zhu: Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China; Corresponding authors.
Lihua Chen: School of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510180, China; Guangzhou Laboratory, Guangzhou 510006, China; Corresponding authors.
Huisheng Liu: School of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510180, China; Guangzhou Laboratory, Guangzhou 510006, China; School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 510006, China; College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Corresponding authors.

Journal volume & issue: Vol. 77
p. 103397

Abstract

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Somatostatin (SST)-producing pancreatic delta-cells play an important role in maintaining the balance of insulin and glucagon secretion within the islets. This study aimed to generate a human embryonic stem cell (hESC) line with a SST-P2A-mCherry reporter using CRISPR/Cas9 system. The SST-P2A-mCherry reporter cell line was shown to maintain typical pluripotent characteristics and able to be induced into SST-producing pancreatic delta-cells. The generation of the cell line would provide useful platform for the characterization of stem cell-derived delta-cells, discovery of delta-cell surface markers and investigation of paracrine mechanisms, which will ultimately promote the drug discovery and cell therapy of diabetes mellitus.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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