Global fitting for high-accuracy multi-channel single-molecule localization

Yiming Li; Wei Shi; Sheng Liu; Ivana Cavka; Yu-Le Wu; Ulf Matti; Decheng Wu; Simone Koehler; Jonas Ries

doi:10.1038/s41467-022-30719-4

Nature Communications (Jun 2022)

Global fitting for high-accuracy multi-channel single-molecule localization

Yiming Li,
Wei Shi,
Sheng Liu,
Ivana Cavka,
Yu-Le Wu,
Ulf Matti,
Decheng Wu,
Simone Koehler,
Jonas Ries

Affiliations

Yiming Li: Department of Biomedical Engineering, Southern University of Science and Technology
Wei Shi: Department of Biomedical Engineering, Southern University of Science and Technology
Sheng Liu: European Molecular Biology Laboratory, Cell Biology and Biophysics
Ivana Cavka: European Molecular Biology Laboratory, Cell Biology and Biophysics
Yu-Le Wu: European Molecular Biology Laboratory, Cell Biology and Biophysics
Ulf Matti: European Molecular Biology Laboratory, Cell Biology and Biophysics
Decheng Wu: Department of Biomedical Engineering, Southern University of Science and Technology
Simone Koehler: European Molecular Biology Laboratory, Cell Biology and Biophysics
Jonas Ries: European Molecular Biology Laboratory, Cell Biology and Biophysics

DOI: https://doi.org/10.1038/s41467-022-30719-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

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Multi-channel SMLM imaging is powerful. Here the authors report globLoc, a GPU-based global fitting algorithm, to extract maximum information from multichannel single molecule data; this gives improved localisation precision for biplane and 4Pi-SMLM and colour assignment in multi-colour astigmatic SMLM.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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