Poly-ε-caprolactone Nanoparticles Loaded with 4-Nerolidylcatechol (4-NC) for Growth Inhibition of <i>Microsporum canis</i>
Vanessa Raquel Greatti,
Fernando Oda,
Rodrigo Sorrechia,
Bárbara Regina Kapp,
Carolina Manzato Seraphim,
Ana Carolina Villas Bôas Weckwerth,
Marlus Chorilli,
Patrícia Bento Da Silva,
Josimar O. Eloy,
Marcelo J. Kogan,
Javier O. Morales,
Rosemeire Cristina Linhari Rodrigues Pietro
Affiliations
Vanessa Raquel Greatti
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Fernando Oda
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Rodrigo Sorrechia
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Bárbara Regina Kapp
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Carolina Manzato Seraphim
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Ana Carolina Villas Bôas Weckwerth
Department of Mycology, Lauro de Souza Lima Institute, Bauru, SP 17034-971, Brazil
Marlus Chorilli
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Patrícia Bento Da Silva
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Josimar O. Eloy
Department of Pharmacy, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, University Avenue, 2853 Rodolfo Teófilo, Fortaleza, CE 60430160, Brazil
Marcelo J. Kogan
School of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, Independencia, Santiago 8380492, Chile
Javier O. Morales
School of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, Independencia, Santiago 8380492, Chile
Rosemeire Cristina Linhari Rodrigues Pietro
Department of Drugs and Medicines, School of Pharmaceutical Sciences, School of Pharmaceutical Sciences of São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, Araraquara, SP 14800-903, Brazil
Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound’s stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC.