Three-dimensional visualizations from a dataset of immunohistochemical stained serial sections of human brain tissue containing tuberculosis related granulomas
Stefan-Dan Zaharie,
Daniel J. Franken,
Martijn van der Kuip,
Sabine van Elsland,
Bernadette S. de Bakker,
Jaco Hagoort,
Sanna L. Roest,
Carmen S. van Dam,
Carlie Timmers,
Regan Solomons,
Ronald van Toorn,
Mariana Kruger,
A. Marceline van Furth
Affiliations
Stefan-Dan Zaharie
Department of Anatomical Pathology, Faculty of Medicine and Health Sciences, Stellenbosch University and National Health Laboratory Services, Francie Van Zijl Dr, Parrow, Tygerberg Hospital, Cape Town 7505, South Africa
Daniel J. Franken
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands
Martijn van der Kuip
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands; Corresponding author.
Sabine van Elsland
Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Dr, Parrow, Tygerberg Hospital, Cape Town 7505, South Africa
Bernadette S. de Bakker
Department of Medical Biology, Section Clinical Anatomy & Embryology, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam Zuidoost, the Netherlands
Jaco Hagoort
Department of Medical Biology, Section Clinical Anatomy & Embryology, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam Zuidoost, the Netherlands
Sanna L. Roest
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands
Carmen S. van Dam
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands
Carlie Timmers
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands
Regan Solomons
Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Dr, Parrow, Tygerberg Hospital, Cape Town 7505, South Africa
Ronald van Toorn
Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Dr, Parrow, Tygerberg Hospital, Cape Town 7505, South Africa
Mariana Kruger
Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Dr, Parrow, Tygerberg Hospital, Cape Town 7505, South Africa
A. Marceline van Furth
Department of Paediatric Infectious Diseases and Immunology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands
This data article presents datasets associated with the research article entitled “The immunological architecture of granulomatous inflammation in central nervous system tuberculosis’’ (Zaharie et al., 2020). The morphology of tuberculosis related granulomas within the central nervous system of human patients was visualized in six different three-dimensional (3D) models. Post-mortem, formalin fixed and paraffin embedded specimens from deceased tuberculous meningitis patients were immunohistochemically stained and 800 serial histologically stained sections were acquired. Images from all sections were obtained with an Olympus BX43 light microscope and structures were identified, labeled and made three-dimensional. The interactive 3D-models allows the user to directly visualize the morphology of the granulomas and to understand the localization of the granulomas. The 3D-models can be used for multiple purposes and provide both an educational source as a gold standard for further animal studies, human research and the development of in silico models on the topic of central nervous system tuberculosis.
