Emerging Infectious Diseases (Dec 2016)

Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

  • David Polo,
  • Julien Schaeffer,
  • Nelly Fournet,
  • Jean-Claude Le Saux,
  • Sylvain Parnaudeau,
  • Catherine McLeod,
  • Françoise S. Le Guyader

DOI
https://doi.org/10.3201/eid2212.160841
Journal volume & issue
Vol. 22, no. 12
pp. 2189 – 2191

Abstract

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Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.

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