STUDIES REGARDING THE VIABILITY OF MOUSE EMBRYOS RECOVERED IN EARLY DEVELOPMENTAL STAGES

Abstract

In order to evaluate the embryo viability we tested 2 cultivation media in three combinations (M16, Nutrient mixture F- 12 HAM supplemented with BSA and Nutrient mixture F- 12 HAM without BSA). In the third day of cultivation the embryos viability was assessed by morphological criteria and randomly by staining with fluorescents dyes (Fluorescein diacetate and Propidium iodide). It was observed that both media used for in vitro cultivation assured embryo development up to the expanded blastocyst stage (21.33% for M 16 and 4.34% respectively), exception the Nutrient mixture F- 12 HAM without BSA in which after 3 days cultivation all embryos were degenerated. The M16 medium assured the embryos development up to the hatched blastocyst stage (5.33%). None of the media supported the development of embryos under 8 cell stages and early blockage in stage of 2 cells could not be prevented.

Keywords

• embryo
• viability
• culture medium
• fad
• pi