Dexamethasone does not ameliorate gliosis in a mouse model of neurodegenerative disease

Xiaolian Ye; Gang Zou; Jinxing Hou; Huiru Bi; Cuihua Zhou; Runmin Wang; Yun Xu; Chun Wang; Guiquan Chen; Zhenyu Yin; Jinping Zhang; Chaoli Huang

Biochemistry and Biophysics Reports (Dec 2020)

Dexamethasone does not ameliorate gliosis in a mouse model of neurodegenerative disease

Xiaolian Ye,
Gang Zou,
Jinxing Hou,
Huiru Bi,
Cuihua Zhou,
Runmin Wang,
Yun Xu,
Chun Wang,
Guiquan Chen,
Zhenyu Yin,
Jinping Zhang,
Chaoli Huang

Affiliations

Xiaolian Ye: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Gang Zou: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Jinxing Hou: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Huiru Bi: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Cuihua Zhou: Department of Anesthesiology, The Second Affiliated Changzhou People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, 213000, China
Runmin Wang: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Yun Xu: Department of Neurology, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, Nanjing University Medical School, 321 Zhongshan Avenue, Nanjing, Jiangsu Province, 210008, China
Chun Wang: Department of Geriatric, Nanjing Drum Tower Hospital, Nanjing University Medical School, 321 Zhongshan Avenue, Nanjing, Jiangsu Province, 210008, China
Guiquan Chen: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China
Zhenyu Yin: Department of Geriatric, Nanjing Drum Tower Hospital, Nanjing University Medical School, 321 Zhongshan Avenue, Nanjing, Jiangsu Province, 210008, China; Corresponding author.
Jinping Zhang: Department of Medicament, Nanjing Drum Tower Hospital, Nanjing University Medical School, 321 Zhongshan Avenue, Nanjing, Jiangsu Province, 210008, China; Corresponding author.
Chaoli Huang: School of Medicine, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 12 Xuefu Avenue, Nanjing, Jiangsu Province, 210061, China; Corresponding author.

Journal volume & issue: Vol. 24
p. 100817

Abstract

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Prolonged neuroinflammation is a driving force for neurodegenerative disease, and agents against inflammatory responses are regarded as potential treatment strategies. Here we aimed to evaluate the prevention effects on gliosis by dexamethasone (DEX), an anti-inflammation drug. We used DEX to treat the nicastrin conditional knockout (cKO) mouse, a neurodegenerative mouse model. DEX (10 mg/kg) was given to 2.5-month-old nicastrin cKO mice, which have not started to display neurodegeneration and gliosis, for 2 months. Immunohistochemistry (IHC) and Western blotting techniques were used to detect changes in neuroinflammatory responses. We found that activation of glial fibrillary acidic protein (GFAP) positive or ionized calcium binding adapter molecule1 (Iba1) positive cells was not inhibited in nicastrin cKO mice treated with DEX as compared to those treated with saline. These data suggest that DEX does not prevent or ameliorate gliosis in a neurodegenerative mouse model when given prior to neuronal or synaptic loss.

Published in Biochemistry and Biophysics Reports

ISSN: 2405-5808 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General); Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.journals.elsevier.com/biochemistry-and-biophysics-reports/

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