Gas–liquid chromatography of hyocholic acid

Abstract

Partial derivatives of hyocholic acid were formed under the usual conditions for trifluoroacetylation of bile acids (trifluoroacetic anhydride, 35°C for 20 min). Complete trifluoroacetylation of hyocholic acid was achieved at 80°C for 30 min, or at 60°C for 30 min when a trace of pyridine was added to the reaction mixture.

Keywords

• bile acids
• methyl esters
• trifluoroacetylation
• partial derivatives
• steric hindrance
• pyridine