Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture

Zixuan Yuan; Mahmud Arif Pavel; Hao Wang; Jerome C. Kwachukwu; Sonia Mediouni; Joseph Anthony Jablonski; Kendall W. Nettles; Chakravarthy B. Reddy; Susana T. Valente; Scott B. Hansen

doi:10.1038/s42003-022-03841-8

Communications Biology (Sep 2022)

Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture

Zixuan Yuan,
Mahmud Arif Pavel,
Hao Wang,
Jerome C. Kwachukwu,
Sonia Mediouni,
Joseph Anthony Jablonski,
Kendall W. Nettles,
Chakravarthy B. Reddy,
Susana T. Valente,
Scott B. Hansen

Affiliations

Zixuan Yuan: Department of Molecular Medicine, The Scripps Research Institute
Mahmud Arif Pavel: Department of Molecular Medicine, The Scripps Research Institute
Hao Wang: Department of Molecular Medicine, The Scripps Research Institute
Jerome C. Kwachukwu: Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Sonia Mediouni: Department of Immunology and Microbiology, The Scripps Research Institute
Joseph Anthony Jablonski: Department of Immunology and Microbiology, The Scripps Research Institute
Kendall W. Nettles: Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Chakravarthy B. Reddy: Department of Internal Medicine, University of Utah Health Sciences Center
Susana T. Valente: Department of Immunology and Microbiology, The Scripps Research Institute
Scott B. Hansen: Department of Molecular Medicine, The Scripps Research Institute

DOI: https://doi.org/10.1038/s42003-022-03841-8
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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Super-resolution microscopy in cultured cells is employed to dissect the effect of hydroxychloroquine (HCQ) at the plasma membrane and HCQ directly perturbs clustering of the SARS-CoV-2 receptor ACE2 with endocytic lipids and PIP2 clusters.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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