In-Situ Observation of New Extra-Vascular Threadlike Structure of Mouse Using a Fluorescence Stereoscopic Microscope

Abstract

We report the new threadlike structures outside the blood vessels of mice. For this, we developed an in-situ searching method of the structure by vital staining with the dye of acridine orange and using a fluorescent stereomicroscope designed specifically for this purpose. We consider that the newly found threadlike structure might be rediscovery of the extra-vascular Bonghan duct which was reported in 1963 by Bonghan Kim.

Keywords

• in-situ
• fluorescence stereoscopic microscope
• acridine orange
• threadlike structure
• extra-vascular Bonghan duct