Lectins use for receive and study of glioma C6 cells with stem cells properties

Abstract

The purpose — to enrich suspension of glioma C6 cells in rats with stem tumor stem cells using lectins sorbed on plastic.Materials and methods. Lectins of beans (Phaseolus vulgaris — PHA), concanavalin (ConA), peanuts (peanut agglutinin — PNA), mistletoe (Mil), labrum anagyroides agglutinin (LABA), soybean (SBA) were used to separate glioma C6 cells.Glioma C6 cells were cultivated with sorbed lectins for 24 h and were injected into the brain of the rats.Results. Tumorogenic activity was significantly higher in fractions of cells adhered to plastic with adsorbed lectins PHA, ConA, PNA, LABA and SBA, that was an indirect evidence of these fractions enriched by tumor stem cells. Lectin Mil exert an antitumor effect that contributed to rats’ lifetime increasing.Conclusions. The results of the study indicate the possibility of tumor cells division using lectins.