Methods for determining Cytochrome P450 reductase in biological fluids of animals

Abstract

The study provides a literature review of modern methods for determining Cytochrome P450 reductase. The importance of the enzyme in the body is established. It is shown that Cytochrome P450 reductase determination is used in pharmacology and toxicology. Observed that modern approaches to determining Cytochrome P450 reductase include mass spectrometry, chromatography, electrochemical methods. Spectrometry – allows detection of low concentrations of the substance and study of reaction kinetics. Chromatography (gas, liquid, thin-layer chromatography) is used to separate complex mixtures and determine enzyme activity. Electrochemical methods (voltammetry) help to study the redox transitions of the enzyme. It is confirmed that ELISA is a new era for the Cytochrome P450 reductase determination. Gaps in modern knowledge about Cytochrome P450 reductase are also identified.