Nature Communications (Apr 2020)
The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis
- Cyril Addi,
- Adrien Presle,
- Stéphane Frémont,
- Frédérique Cuvelier,
- Murielle Rocancourt,
- Florine Milin,
- Sandrine Schmutz,
- Julia Chamot-Rooke,
- Thibaut Douché,
- Magalie Duchateau,
- Quentin Giai Gianetto,
- Audrey Salles,
- Hervé Ménager,
- Mariette Matondo,
- Pascale Zimmermann,
- Neetu Gupta-Rossi,
- Arnaud Echard
Affiliations
- Cyril Addi
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Adrien Presle
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Stéphane Frémont
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Frédérique Cuvelier
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Murielle Rocancourt
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Florine Milin
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Sandrine Schmutz
- Institut Pasteur, UTechS CB
- Julia Chamot-Rooke
- Institut Pasteur, Mass Spectrometry for Biology Unit, C2RT, USR 2000, CNRS
- Thibaut Douché
- Institut Pasteur, Proteomics Platform, Mass Spectrometry for Biology, C2RT, USR 2000, CNRS
- Magalie Duchateau
- Institut Pasteur, Proteomics Platform, Mass Spectrometry for Biology, C2RT, USR 2000, CNRS
- Quentin Giai Gianetto
- Institut Pasteur, Proteomics Platform, Mass Spectrometry for Biology, C2RT, USR 2000, CNRS
- Audrey Salles
- UTechS Photonic BioImaging PBI (Imagopole), Centre de Recherche et de Ressources Technologiques C2RT, Institut Pasteur
- Hervé Ménager
- Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS
- Mariette Matondo
- Institut Pasteur, Proteomics Platform, Mass Spectrometry for Biology, C2RT, USR 2000, CNRS
- Pascale Zimmermann
- Centre de Recherche en Cancérologie de Marseille (CRCM), Equipe labellisée Ligue 2018, Aix-Marseille Université, Inserm, CNRS, Institut Paoli Calmettes
- Neetu Gupta-Rossi
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- Arnaud Echard
- Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS
- DOI
- https://doi.org/10.1038/s41467-020-15205-z
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
ESCRT filaments drive the final abscission between two daughter cells but how they physically interact with the membrane is unclear. Using proteomics, the authors show that syndecan-4/syntenin/ALIX couples the ESCRT machinery to the abscission site and thus promotes efficient abscission.
