Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics
Camilla Marasca,
Maria Encarnacion Blanco Arana,
Michele Protti,
Andrea Cavalli,
Laura Mercolini,
Andrea Armirotti
Affiliations
Camilla Marasca
Computational and Chemical Biology, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
Maria Encarnacion Blanco Arana
Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
Michele Protti
Research Group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
Andrea Cavalli
Computational and Chemical Biology, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
Laura Mercolini
Research Group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
Andrea Armirotti
Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.
