Engineered Regeneration (Jun 2023)
Conductive PS inverse opals for regulating proliferation and differentiation of neural stem cells
- Yangnan Hu,
- Han Zhang,
- Hao Wei,
- Menghui Liao,
- Xiaoyan Chen,
- Jiayue Xing,
- Lian Duan,
- Cuntu Cheng,
- Weicheng Lu,
- Xuechun Yang,
- Peina Wu,
- Huan Wang,
- Jingdun Xie,
- Renjie Chai
Affiliations
- Yangnan Hu
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
- Han Zhang
- State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
- Hao Wei
- Department of Otolaryngology Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical School Jiangsu Provincial Key Medical Discipline, Nanjing 210008, China
- Menghui Liao
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
- Xiaoyan Chen
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China
- Jiayue Xing
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China
- Lian Duan
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China
- Cuntu Cheng
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China
- Weicheng Lu
- Department of Anesthesiology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in Southern China, Collaborative Innovation for Cancer Medicine, Guangdong 510060, China
- Xuechun Yang
- Department of Otolaryngology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; School of Medicine, South China University of Technology, Guangzhou, 510006, China
- Peina Wu
- Department of Otolaryngology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; School of Medicine, South China University of Technology, Guangzhou, 510006, China; Corresponding authors.
- Huan Wang
- The Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen, 518033, China; Corresponding authors.
- Jingdun Xie
- Department of Anesthesiology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in Southern China, Collaborative Innovation for Cancer Medicine, Guangdong 510060, China; Corresponding authors.
- Renjie Chai
- State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China; Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, 100069, China; Corresponding authors.
- Journal volume & issue
-
Vol. 4,
no. 2
pp. 214 – 221
Abstract
The development of neural tissue engineering has brought new hope to the treatment of spinal cord injury (SCI). Up to date, various scaffolds have been developed to induce the oriented growth and arrangement of nerves to facilitate the repair after injury. In this work, a conductive and anisotropic inverse opal substrate was presented by modifying polystyrene (PS) inverse opal films with carbon nanotubes and then stretching them to varying degrees. The film had good biocompatibility, and neural stem cells (NSCs) grown on the film displayed good orientation along the stretching direction. In addition, benefiting from the conductivity and anisotropy of the film, NSCs differentiated into neurons significantly. These results suggest that the conductive and anisotropic PS inverse opal substrates possess value in nerve tissue engineering regeneration.
