Antioxidative and Metabolic Contribution to Salinity Stress Responses in Two Rapeseed Cultivars during the Early Seedling Stage
Ali Mahmoud El-Badri,
Maria Batool,
Ibrahim A. A. Mohamed,
Zongkai Wang,
Ahmed Khatab,
Ahmed Sherif,
Hasan Ahmad,
Mohammad Nauman Khan,
Hamada Mohamed Hassan,
Ibrahim M. Elrewainy,
Jie Kuai,
Guangsheng Zhou,
Bo Wang
Affiliations
Ali Mahmoud El-Badri
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Maria Batool
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Ibrahim A. A. Mohamed
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Zongkai Wang
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Ahmed Khatab
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Ahmed Sherif
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Hasan Ahmad
National Gene Bank, Agricultural Research Center (ARC), Giza 12619, Egypt
Mohammad Nauman Khan
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Hamada Mohamed Hassan
Field Crops Research Institute, Agricultural Research Center (ARC), Giza 12619, Egypt
Ibrahim M. Elrewainy
Field Crops Research Institute, Agricultural Research Center (ARC), Giza 12619, Egypt
Jie Kuai
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Guangsheng Zhou
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Bo Wang
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Measuring metabolite patterns and antioxidant ability is vital to understanding the physiological and molecular responses of plants under salinity. A morphological analysis of five rapeseed cultivars showed that Yangyou 9 and Zhongshuang 11 were the most salt-tolerant and -sensitive, respectively. In Yangyou 9, the reactive oxygen species (ROS) level and malondialdehyde (MDA) content were minimized by the activation of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) for scavenging of over-accumulated ROS under salinity stress. Furthermore, Yangyou 9 showed a significantly higher positive correlation with photosynthetic pigments, osmolyte accumulation, and an adjusted Na+/K+ ratio to improve salt tolerance compared to Zhongshuang 11. Out of 332 compounds identified in the metabolic profile, 225 metabolites were filtrated according to p Brassica napus L. during the early seedling stage is of great reference value for plant breeders to develop salt-tolerant rapeseed cultivars.
