Analysis of damage-associated molecular pattern molecules due to electroporation of cells in vitro

Abstract

Tumor cells can die via immunogenic cell death pathway, in which damage-associated molecular pattern molecules (DAMPs) are released from the cells. These molecules activate cells involved in the immune response. Both innate and adaptive immune response can be activated, causing a destruction of the remaining infected cells. Activation of immune response is also an important component of tumor treatment with electrochemotherapy (ECT) and irreversible electroporation (IRE). We thus explored, if and when specific DAMPs are released as a consequence of electroporation in vitro.

Keywords

• electroporation
• pulsed electric field treatment
• damage-associated molecular pattern molecules
• immunogenic cell death
• electrochemotherapy