International Journal of Nanomedicine (Sep 2022)
In-vivo (Albino Mice) and in-vitro Assimilation and Toxicity of Zinc Oxide Nanoparticles in Food Materials
Abstract
Saiqa Bashir,1 Muhammad Siddique Awan,1 Muhammad Akhyar Farrukh,2,3 Ravi Naidu,4 Shahzad Akbar Khan,5 Nagina Rafique,1 Shaista Ali,6 Imran Hayat,1 Imtiaz Hussain,1 Muhammad Zubair Khan7 1Department of Food Science and Technology, University of Poonch, Rawalakot, Pakistan; 2Department of Chemistry, Forman Christian College (A Chartered University), Lahore, Pakistan; 3Department of Basic and Applied Chemistry, University of Central Punjab, Lahore, Pakistan; 4Global Centre for Environmental Remediation (GCER), University of Newcastle, Newcastle, Australia; 5Department of Pathobiology, Faculty of Veterinary & Animal Sciences, University of Poonch, Rawalakot, Pakistan; 6Department of Chemistry, Government College University of Lahore, Lahore, Pakistan; 7Department of Plant Breeding and Molecular Genetics, University of Poonch, Rawalakot, PakistanCorrespondence: Saiqa Bashir, Email [email protected]: Recent advances in nanotechnology have given rise to the potential utilization of nanoparticles as food, nano-medicine/biomedicines.Patient: The study aimed to investigate the effects of nano-zinc oxide (nano-zinc) on the bio-assimilation of mineral (Zn) in mice, aged 3– 6 weeks.Methods: ZnO nanoparticles were added to the basal diet as a supplement at amounts of 0.07, 0.14 and 0.21 mg/kg. The synthesized material was characterized by Fourier transform infrared spectrophotometer, particle size, scanning electron microscope, Thermogravimetric Analysis Thermal, X-ray diffraction spectrophotometer and Zeta potential.Results: In-vitro bioavailability of synthesized group ZnO (120 nm) was 43%, whereas for standard group ZnO (50 nm) was reported as 55%. In-vivo bioavailability of zinc oxide illustrated the maximum absorption level compared with the control. In-vivo toxicity was characterized as damage done to the liver and spleen tissues with a high dose of 0.21 mg/kg, while smaller doses indicated no toxic effects.Conclusion: The study provided important insights on the toxicological effects of ZnO nanoparticles, depending on dose rate and bio-assimilation, as well as particles, under various conditions (in-vitro and in-vivo). These findings will motivate further detailed research on nano-based medicine for alleviating malnutrition conditions.Keywords: bioavailability, toxicity, nanoparticles, nanomedicine, food
