Veterinary Research (Nov 2020)

Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

  • Tianqiong He,
  • Mingshu Wang,
  • Anchun Cheng,
  • Qiao Yang,
  • Renyong Jia,
  • Ying Wu,
  • Juan Huang,
  • Shun Chen,
  • Xin-Xin Zhao,
  • Mafeng Liu,
  • Dekang Zhu,
  • Shaqiu Zhang,
  • Xuming Ou,
  • Sai Mao,
  • Qun Gao,
  • Di Sun,
  • XinJian Wen,
  • Bin Tian,
  • Yunya Liu,
  • Yanling Yu,
  • Ling Zhang,
  • Leichang Pan,
  • Xiaoyue Chen

DOI
https://doi.org/10.1186/s13567-020-00859-w
Journal volume & issue
Vol. 51, no. 1
pp. 1 – 12

Abstract

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Abstract Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).

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