Inmunofenotipaje celular en el diagnostico de la leucemia mieloide aguda Cellular immunophenotyping in the diagnosis of acute myeloid leukemia

Abstract

The immunophenotype of the leukemic cells was analyzed in 72 patients with diagnosis of acute myeloid leukemia (AML) that received attention at the Institute of Hematology and Immunology during 13 years. The cellular immunophenotyping (CIP) was performed by immunocytochemical ultramicromethod, using a panel of monoclonal antibodies to evaluate the expression of myeloid and lymphoid antigens. Of the total of studied AMLs, 22 % corresponded to the varieties M1 and M4. The rest of the diagnosed varieties were: M3 (19.4 %), M0 (15.3 %), M5 (11.1 %), M2 and M7 (4.2 %), and M6 (1.4 %). The expression of lymphoid antigens was found in 31.9 % of these patients. The terminal desoxynucleotidyl transferase was positive in 56.5 % of the cases. The cellular immunophenotyping is a useful method for the diagnosis of varieties M0 and M7 of acute lymphoid leukemia, and it also allows to complement the cytomorphological and cytochemical diagnosis of the rest of the varieties

Keywords

• Acute myeloid leukemia
• immunophenotype
• monoclonal antibody