Nitrogen Advanced Treatment of Urban Sewage by Denitrification Deep-Bed Filter: Removal Performance and Metabolic Pathway

Xiao Huang; Xiao Huang; Yixiao Xing; Hongjie Wang; Hongjie Wang; Zhongyi Dai; Tiantian Chen

doi:10.3389/fmicb.2021.811697

Frontiers in Microbiology (Jan 2022)

Nitrogen Advanced Treatment of Urban Sewage by Denitrification Deep-Bed Filter: Removal Performance and Metabolic Pathway

Xiao Huang,
Xiao Huang,
Yixiao Xing,
Hongjie Wang,
Hongjie Wang,
Zhongyi Dai,
Tiantian Chen

Affiliations

Xiao Huang: Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, China
Xiao Huang: Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, School of Civil and Environmental Engineering, Harbin Institute of Technology, Shenzhen, China
Yixiao Xing: Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, China
Hongjie Wang: Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, School of Civil and Environmental Engineering, Harbin Institute of Technology, Shenzhen, China
Hongjie Wang: State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, China
Zhongyi Dai: China Municipal Engineering Central South Design and Research Institute Co., Ltd., Wuhan, China
Tiantian Chen: CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

DOI: https://doi.org/10.3389/fmicb.2021.811697
Journal volume & issue: Vol. 12

Abstract

Read online

This study aimed to explore the performance of denitrification deep-bed filter (DN-DBF) to treat municipal sewage for meeting a more stringent discharge standard of total nitrogen (TN) (10.0 mg L–1). A lab-scale DN-DBF was conducted to optimize operation parameters and reveal the microbiological mechanism for TN removal. The results showed that more than 12.7% TN removal was obtained by adding methanol compared with sodium acetate. The effluent TN concentration reached 6.0–7.0 mg L–1 with the optimal influent carbon and nitrogen ratio (C/N) and hydraulic retention time (HRT) (3:1 and 0.25 h). For the nitrogen removal mechanism, Blastocatellaceae_Subgroup_4 and norank_o_JG30-KF-CM45 were dominant denitrification floras with an abundance of 6–10%. Though large TN was removed at the top layer of DN-DBF, microbial richness and diversity at the middle layer were higher than both ends. However, the relative abundance of nitrite reductase enzymes (EC1.7.2.1) gradually increases as the depth increases; conversely, the relative abundance of nitrous oxide reductase gradually decreased.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

About the journal

Abstract

Keywords