Circular RNA hsa_circ_0051040 Promotes Hepatocellular Carcinoma Progression by Sponging miR-569 and Regulating ITGAV Expression
Linling Ju,
Min Yao,
Rujian Lu,
Yali Cao,
Huixuan Wang,
Liuxia Yuan,
Feng Xiao,
Jianguo Shao,
Weihua Cai,
Lin Chen,
Zhaolian Bian
Affiliations
Linling Ju
Medical School of Nantong University, Nantong University, Nantong 226001, China
Min Yao
Medical School of Nantong University, Nantong University, Nantong 226001, China
Rujian Lu
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Yali Cao
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Huixuan Wang
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Liuxia Yuan
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Feng Xiao
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Jianguo Shao
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Weihua Cai
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Lin Chen
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Zhaolian Bian
Nantong Institute of Liver Disease, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People’s Hospital, Nantong University, Nantong 226006, China
Accumulating evidence has demonstrated the roles of circular RNAs (circRNAs) in hepatocellular carcinoma (HCC); however, their roles in HCC need to be further studied. Through high-throughput human circRNA microarray analysis of HCC and adjacent normal tissues, we identified hsa_circ_0051040 as a novel candidate circRNA for the diagnosis and treatment of HCC. In this study, we found that hsa_circ_0051040 was overexpressed in HCC tissues and cell lines and that its expression was correlated with poor prognosis. Knockdown of hsa_circ_0051040 inhibited the migration, invasion, and proliferation of HCC cells in vitro and in vivo, whereas overexpression of hsa_circ_0051040 had the opposite effects. Moreover, our data demonstrated that hsa_circ_0051040 acted as a sponge for miR-569 to regulate ITGAV expression and induce EMT progression. Our findings indicated that hsa_circ_0051040 promotes HCC development and progression by sponging miR-569 to increase ITGAV expression. Thus, hsa_circ_0051040 is a good candidate as a therapeutic target.
