Discovery of tumor immune infiltration-related snoRNAs for predicting tumor immune microenvironment status and prognosis in lung adenocarcinoma

Rongjun Wan; Lu Bai; Changjing Cai; Wang Ya; Juan Jiang; Chengping Hu; Qiong Chen; Bingrong Zhao; Yuanyuan Li

Computational and Structural Biotechnology Journal (Jan 2021)

Discovery of tumor immune infiltration-related snoRNAs for predicting tumor immune microenvironment status and prognosis in lung adenocarcinoma

Rongjun Wan,
Lu Bai,
Changjing Cai,
Wang Ya,
Juan Jiang,
Chengping Hu,
Qiong Chen,
Bingrong Zhao,
Yuanyuan Li

Affiliations

Rongjun Wan: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Lu Bai: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Changjing Cai: National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Wang Ya: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Juan Jiang: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Chengping Hu: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Qiong Chen: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Bingrong Zhao: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008
Yuanyuan Li: Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China, 410008; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. 410008; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan, China. 410008; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan, China. 410008; National Clinical Research Center for Geriatric Disorders，Xiangya Hospital, Changsha, Hunan, P.R. China, 410008; Corresponding author.

Journal volume & issue: Vol. 19
pp. 6386 – 6399

Abstract

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Lung adenocarcinoma (LUAD) has a high mortality rate and is difficult to diagnose and treat in its early stage. Previous studies have demonstrated that small nucleolar RNAs (snoRNAs) play a critical role in tumor immune infiltration and the development of a variety of solid tumors. However, there have been no studies on the correlation between tumor-infiltrating immune-related snoRNAs (TIISRs) and LUAD.In this study, we filtered six immune-related snoRNAs based on the tissue specificity index (TSI) and expression profile of all snoRNAs between all LUAD cell lines from the Cancer Cell Line Encyclopedia and 21 types of immune cells from the Gene Expression Omnibus database. Further, we performed real-time quantitative polymerase chain reaction (RT-qPCR) to validate the expression status of these snoRNAs on peripheral blood mononuclear cells (PBMCs) and lung cancer cell lines. Next, we developed a TIISR signature based on the expression profiles of snoRNAs from 479 LUAD patients filtered by the random survival forest algorithm. We then analyzed the value of this TIISR signature (TIISR risk score) for assessing tumor immune infiltration, immune checkpoint inhibitor (ICI) treatment response, and the prognosis of LUAD between groups with high and low TIISR risk score. Further, we found that the TIISR risk score groups showed significant differences in biological characteristics and that the risk score could be used to assess the level of tumor immune cell infiltration, thereby predicting prognosis and responsiveness to immunotherapy in LUAD patients.

Published in Computational and Structural Biotechnology Journal

ISSN: 2001-0370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/computational-and-structural-biotechnology-journal

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