The Modification of H3K4me3 Enhanced the Expression of <i>Cg</i>TLR3 in Hemocytes to Increase <i>Cg</i>IL17-1 Production in the Immune Priming of <i>Crassostrea gigas</i>

Abstract

Increasing evidence confirms that histone modification plays a critical role in preserving long-term immunological memory. Immune priming is a novel form of immunological memory recently verified in invertebrates. Toll-like receptor (TLR) signaling and cytokines have been reported to be involved in the immune priming of the Pacific oyster Crassostrea gigas. In the present study, the expression of Toll-like receptor 3 (CgTLR3), myeloid differentiation factor 88-2 (CgMyd88-2) and interleukin 17-1 (CgIL17-1) was found to be elevated in the hemocytes of C. gigas at 6 h after the secondary stimulation with Vibrio splendidus, which was significantly higher than that at 6 h after the primary stimulation (p CgTLR3 gene at 7 d after the primary stimulation with inactivated V. splendidus (p CgTLR3 gene decreased significantly at 7 d after the primary stimulation with inactivated V. splendidus (p CgTLR3, CgMyD88-2 and CgIL17-1 was significantly repressed at 6 h after the secondary stimulation with V. splendidus (p CgTLR3 promoter at 7 d after the primary stimulation (p CgTLR3, CgMyD88-2 and CgIL17-1 was observed to increase significantly at 6 h after the secondary stimulation (p C. gigas, which defined the role of histone modifications in invertebrate immune priming.

Keywords

• H3K4me3
• immune priming
• interleukin 17
• Toll-like receptor
• <i>Crassostrea gigas</i>