Journal of Hematology & Oncology (Feb 2021)
Clonal evolution in liver cancer at single-cell and single-variant resolution
- Xianbin Su,
- Linan Zhao,
- Yi Shi,
- Rui Zhang,
- Qi Long,
- Shihao Bai,
- Qing Luo,
- Yingxin Lin,
- Xin Zou,
- Shila Ghazanfar,
- Kun Tao,
- Guoliang Yang,
- Lan Wang,
- Kun-Yan He,
- Xiaofang Cui,
- Jian He,
- Jiao-Xiang Wu,
- Bo Han,
- Bin Yan,
- Biao Deng,
- Na Wang,
- Xiaolin Li,
- Pengyi Yang,
- Shangwei Hou,
- Jielin Sun,
- Jean Y. H. Yang,
- Jinhong Chen,
- Ze-Guang Han
Affiliations
- Xianbin Su
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Linan Zhao
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Yi Shi
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Shanghai Jiao Tong University
- Rui Zhang
- Department of General Surgery, Huashan Hospital and Cancer Metastasis Institute, Fudan University
- Qi Long
- Key Laboratory for Regenerative Medicine (Ministry of Education), School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong
- Shihao Bai
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Qing Luo
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Yingxin Lin
- School of Mathematics and Statistics and Charles Perkins Center, The University of Sydney
- Xin Zou
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Shila Ghazanfar
- Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge
- Kun Tao
- Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Guoliang Yang
- Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine
- Lan Wang
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Kun-Yan He
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Xiaofang Cui
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Jian He
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Jiao-Xiang Wu
- Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Bo Han
- Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Bin Yan
- Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
- Biao Deng
- Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
- Na Wang
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Xiaolin Li
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Pengyi Yang
- School of Mathematics and Statistics and Charles Perkins Center, The University of Sydney
- Shangwei Hou
- Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Jielin Sun
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Jean Y. H. Yang
- School of Mathematics and Statistics and Charles Perkins Center, The University of Sydney
- Jinhong Chen
- Department of General Surgery, Huashan Hospital and Cancer Metastasis Institute, Fudan University
- Ze-Guang Han
- Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- DOI
- https://doi.org/10.1186/s13045-021-01036-y
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 5
Abstract
Abstract Genetic heterogeneity of tumor is closely related to its clonal evolution, phenotypic diversity and treatment resistance, and such heterogeneity has only been characterized at single-cell sub-chromosomal scale in liver cancer. Here we reconstructed the single-variant resolution clonal evolution in human liver cancer based on single-cell mutational profiles. The results indicated that key genetic events occurred early during tumorigenesis, and an early metastasis followed by independent evolution was observed in primary liver tumor and intrahepatic metastatic portal vein tumor thrombus. By parallel single-cell RNA-Seq, the transcriptomic phenotype of HCC was found to be related with genetic heterogeneity. For the first time we reconstructed the single-cell and single-variant clonal evolution in human liver cancer, and dissection of both genetic and phenotypic heterogeneity will facilitate better understanding of their relationship.
Keywords
