Food and Agricultural Immunology (Jan 2018)

Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

  • Lingling Guo,
  • Liguang Xu,
  • Shanshan Song,
  • Liqiang Liu,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1401045
Journal volume & issue
Vol. 29, no. 1
pp. 458 – 469

Abstract

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Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 1010 l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, a gold immunochromatographic assay was developed to detect MD residues in chicken breast and egg samples. For semi-quantitative detection by the naked eye, the visual limit of detection was 5 ng/g in chicken breast, 10 ng/g in egg. Quantitative results can be obtained by a hand-held strip scan reader, with the detection range of 5.11–19.34 ng/g in chicken breast and 6.46–27.87 ng/g in egg. The strip test took 10 min to run in total. This strip assay is suitable for on-site detection of MD residues in chicken breast and egg samples.

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