Hepatoprotective Effect of Aqueous Neem Leaves Extract on Hepatocytes and Nuclear Diameter of Albino Wistar Rats Induced with Erythromycin Toxicity

Abstract

Objective: To evaluate the hepatoprotective effects of Neem leaves extract on hepatocyte and nuclear diameters of Albino Wistar rats induced by erythromycin hepatotoxicity. Study Design: Laboratory-based Experimental study. Place and Duration of Study: The study was carried out at the Department of Anatomy and Animal House of Baqai Medical University (BMU) in Karachi, Pakistan from January 2019 to March 2019. Methods: A total of 80 Albino Wistar rats were divided equally into 4 groups. The control group was marked as Group A with no interventions. In Group B only erythromycin was administered. For Group C both erythromycin and aqueous Neem leave Extract were given and for Group D only aqueous neem leave was given. Animals were sacrificed and the liver weight of Albino Wistar rats was measured. Results: It was observed that rats of experimental control group A noticed no significant change in hepatocytes13.19± 0.44μm and nuclear diameters 7.18±0. 28μm (Figure.1) as this group had no intervention received. In group B hepatocyte diameter markedly increased by 16.54±0.6μm and a decrease in nuclear diameter of 5.20±0.30μm (p<0.01) with loss of architecture, ballooning, and edematous changes (Figure.2, 3 and 4) was observed as this group received only erythromycin while group C received both erythromycin and aqueous Neem leaf extract showed histological architecture disrupted to some extent and hepatocytes 14.49±80μm and nuclear diameter 6.13±0.17μm were started to improve its normal sizes (Figure.5). Group D observed no significant changes in hepatocytes 13.0±0.52μm and nuclear diameters 7.14±0.30μm as this group only received aqueous neem leave extract (Figure.6). Conclusion: According to the current study, Erythromycin exerts oxidative stress and Changes in hepatocytes and nuclear diameters were noticeably improved by aqueous neem leaf extract, which is a powerful antioxidant. How to cite this: Jamshed N, Raza I, Razzaq M, Jamshed N, Rasheed A, Faisal L. Hepatoprotective Effect of Aqueous Neem Leaves Extract on Hepatocytes and Nuclear Diameter of Albino Wistar Rats Induced with Erythromycin Toxicity. Life and Science. 2024; 5(2): 228-236. doi: http://doi.org/10.37185/LnS.1.1.572

Keywords

• erythromycin, hemorrhages, hepatocytes, oxidative stress