Nature Communications (Dec 2017)
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
- Qianxin Wu,
- Quentin R. V. Ferry,
- Toni A. Baeumler,
- Yale S. Michaels,
- Dimitrios M. Vitsios,
- Omer Habib,
- Roland Arnold,
- Xiaowei Jiang,
- Stefano Maio,
- Bruno R. Steinkraus,
- Marta Tapia,
- Paolo Piazza,
- Ni Xu,
- Georg A. Holländer,
- Thomas A. Milne,
- Jin-Soo Kim,
- Anton J. Enright,
- Andrew R. Bassett,
- Tudor A. Fulga
Affiliations
- Qianxin Wu
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Quentin R. V. Ferry
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Toni A. Baeumler
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Yale S. Michaels
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Dimitrios M. Vitsios
- European Molecular Biology Laboratory-European Bioinformatics Institute
- Omer Habib
- Center for Genome Engineering, Institute for Basic Science (IBS)
- Roland Arnold
- Molecular and Population Genetics Laboratory, Oxford Centre for Cancer Gene Research, Wellcome Trust Centre for Human Genetics, University of Oxford
- Xiaowei Jiang
- Molecular and Population Genetics Laboratory, Oxford Centre for Cancer Gene Research, Wellcome Trust Centre for Human Genetics, University of Oxford
- Stefano Maio
- Weatherall Institute of Molecular Medicine, Developmental Immunology, University of Oxford
- Bruno R. Steinkraus
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Marta Tapia
- Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford
- Paolo Piazza
- Wellcome Trust Centre for Human Genetics
- Ni Xu
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- Georg A. Holländer
- Weatherall Institute of Molecular Medicine, Developmental Immunology, University of Oxford
- Thomas A. Milne
- Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford
- Jin-Soo Kim
- Center for Genome Engineering, Institute for Basic Science (IBS)
- Anton J. Enright
- European Molecular Biology Laboratory-European Bioinformatics Institute
- Andrew R. Bassett
- Sir William Dunn School of Pathology, University of Oxford
- Tudor A. Fulga
- Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
- DOI
- https://doi.org/10.1038/s41467-017-00686-2
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 17
Abstract
RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in situ analysis of native RREs.
