Acta Veterinaria (Mar 2022)

Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

  • Galecio Juan Sebastián,
  • Hernandis Verónica,
  • Escudero Elisa,
  • Marín Pedro

DOI
https://doi.org/10.2478/acve-2022-0008
Journal volume & issue
Vol. 72, no. 1
pp. 100 – 110

Abstract

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A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times were 3.0 min and 6.4 min for tildipirosin and tylosin tartrate, respectively. The total run time was 9 minutes in this method.

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