Проблемы особо опасных инфекций (Jun 2014)

Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

  • S. V. Generalov,
  • E. G. Abramova,
  • Zh. V. Matveeva,
  • I. M. Zhulidov,
  • L. V. Savitskaya,
  • O. A. Lobovikova

DOI
https://doi.org/10.21055/0370-1069-2014-2-101-103
Journal volume & issue
Vol. 0, no. 2
pp. 101 – 103

Abstract

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/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum.

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