Dilute-and-Shoot-Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry for Pteridine Profiling in Human Urine and Its Association with Different Pathologies
Claudia Giménez-Campillo,
Marta Pastor-Belda,
Natalia Arroyo-Manzanares,
Natalia Campillo,
Blanca del Val Oliver,
José Zarauz-García,
Luis Sáenz,
Pilar Viñas
Affiliations
Claudia Giménez-Campillo
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Regional Campus of International Excellence “Campus Mare Nostrum”, E-30071 Murcia, Spain
Marta Pastor-Belda
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Regional Campus of International Excellence “Campus Mare Nostrum”, E-30071 Murcia, Spain
Natalia Arroyo-Manzanares
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Regional Campus of International Excellence “Campus Mare Nostrum”, E-30071 Murcia, Spain
Natalia Campillo
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Regional Campus of International Excellence “Campus Mare Nostrum”, E-30071 Murcia, Spain
Blanca del Val Oliver
Internal Medicine Service—Gastroenterology and Hepatology Section, Hospital General Universitario Rafael Méndez, 30813 Lorca, Spain
José Zarauz-García
Laboratory Medicine Department, Hospital General Universitario Rafael Méndez, 30813 Lorca, Spain
Luis Sáenz
Laboratory Medicine Department, Hospital General Universitario Rafael Méndez, 30813 Lorca, Spain
Pilar Viñas
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Regional Campus of International Excellence “Campus Mare Nostrum”, E-30071 Murcia, Spain
Pteridines are a group of compounds synthesised by many living organisms that are involved in the metabolism of many cofactors and vitamins. Their concentration in biological fluids may be altered by various pathologies such as cancer or inflammatory bowel disease, urine being the main route of excretion. In this study, three lumazines and ten pterins were analysed in their native oxidation state using high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Their high concentration in urine samples and their good ionisation behaviour allow the use of the dilute-and-shoot method by simple filtration of the urine prior to chromatographic analysis. The final method offers excellent linearity, sensitivity and precision parameters, and a total of 135 urine samples were analysed from patients with some relevant information such as faecal calprotectin (FCP) levels, common diseases such as diabetes, hypertension and dyslipidaemia and immunological diseases such as inflammatory bowel disease (IBD). The pteridine profile was related to FCP levels without showing any correlation. In addition, pteridine levels were compared between healthy subjects and IBD, diabetic, hypertensive and dyslipidaemic patients, and significant differences were found between the two groups for some of the pteridines.
