Nature Communications (Mar 2021)
A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
- Chunyan Wang,
- Rien van Haperen,
- Javier Gutiérrez-Álvarez,
- Wentao Li,
- Nisreen M. A. Okba,
- Irina Albulescu,
- Ivy Widjaja,
- Brenda van Dieren,
- Raul Fernandez-Delgado,
- Isabel Sola,
- Daniel L. Hurdiss,
- Olalekan Daramola,
- Frank Grosveld,
- Frank J. M. van Kuppeveld,
- Bart L. Haagmans,
- Luis Enjuanes,
- Dubravka Drabek,
- Berend-Jan Bosch
Affiliations
- Chunyan Wang
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Rien van Haperen
- Department of Cell Biology, Erasmus Medical Center
- Javier Gutiérrez-Álvarez
- Department of Molecular and Cell Biology, National Center for Biotechnology-Spanish National Research Council (CNB-CSIC)
- Wentao Li
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Nisreen M. A. Okba
- Department of Viroscience, Erasmus Medical Center
- Irina Albulescu
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Ivy Widjaja
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Brenda van Dieren
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Raul Fernandez-Delgado
- Department of Molecular and Cell Biology, National Center for Biotechnology-Spanish National Research Council (CNB-CSIC)
- Isabel Sola
- Department of Molecular and Cell Biology, National Center for Biotechnology-Spanish National Research Council (CNB-CSIC)
- Daniel L. Hurdiss
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Olalekan Daramola
- Cell Culture and Fermentation Sciences, Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca
- Frank Grosveld
- Department of Cell Biology, Erasmus Medical Center
- Frank J. M. van Kuppeveld
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Bart L. Haagmans
- Department of Viroscience, Erasmus Medical Center
- Luis Enjuanes
- Department of Molecular and Cell Biology, National Center for Biotechnology-Spanish National Research Council (CNB-CSIC)
- Dubravka Drabek
- Department of Cell Biology, Erasmus Medical Center
- Berend-Jan Bosch
- Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- DOI
- https://doi.org/10.1038/s41467-021-21968-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Here, the authors report the isolation and characterization of two human monoclonal antibodies (mAbs) from immunized mice with trimeric spike ectodomains of three human betacoronaviruses HCoV-OC43, SARS-CoV and MERSCoV, and show that while exhibiting cross-reactivity, the mAbs only neutralize MERS-CoV but not SARS-CoV nor SARS-CoV-2, likely due to the subtle epitope differences in the spike S2 fusion subunit.
