Nature Communications (Jun 2020)

Multi-site-mediated entwining of the linear WIR-motif around WIPI β-propellers for autophagy

  • Jinqi Ren,
  • Ruobing Liang,
  • Wenjuan Wang,
  • Dachuan Zhang,
  • Li Yu,
  • Wei Feng

DOI
https://doi.org/10.1038/s41467-020-16523-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 9

Abstract

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WIPI proteins are mammalian PROPPIN family phosphoinositide effectors that recognize a WIPI-interacting-region (WIR)-motif to recruit other regulators during autophagosome formation. Here, the authors combine structural and functional studies and present the crystal structure of human WIPI3 with a bound ATG2A WIR-peptide and observe that the WIR-motif binds to three sites in blades 1-3 of WIPI3 and mutating these binding sites impairs the ATG2A-mediated autophagic process.