International Journal of General Medicine (Oct 2023)
KRT19 is a Promising Prognostic Biomarker and Associates with Immune Infiltrates in Serous Ovarian Cystadenocarcinoma
Abstract
Zhe Sun,1,* Ruijie Zhou,2,* Jinjin Dai,2,* Jihua Chen,2 Yu Liu,2 Mengyi Wang,2 Runlong Zhou,2 Fengchen Liu,2 Qinxing Zhang,3 Yao Xu,2 Tongcun Zhang1,2 1College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People’s Republic of China; 2Institute of Biology and Medicine, College of Life Sciences and Health, Wuhan University of Science and Technology, Wuhan, Hubei, People’s Republic of China; 3Wuhan Bio-Raid Biotechnology Co., Ltd, Wuhan, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yao Xu; Tongcun Zhang, Institute of Biology and Medicine, College of Life Science and Health, Wuhan University of Science and Technology, No. 2 Huangjiahu West Road, Wuhan, Hubei, 430081, People’s Republic of China, Tel +86-027-68897343, Fax +86-027-68893590, Email [email protected]; [email protected]: Ovarian cancer (OV) is the highest prevalent gynecologic tumor with complicated pathogenesis; high-grade serous ovarian cystadenocarcinoma (HGSOC) is the most epidemiological and malignant subtype of OV. Keratin type I cytoskeleton 19 (KRT19) is an intermediate filament protein which plays essential roles in the maintenance of epithelial cells. However, its role in OV remains largely unknown.Methods: Bioinformatic analysis with various databases was conducted in this study. In details, KRT19 expression was assessed using databases including The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Omnibus (GEO) and Human Protein Atlas (HPA). GO-KEGG and GSEA analysis were performed by R packages. The biological function of KRT19 was analyzed based on the single-cell sequencing information from CancerSEA database. The association of KRT19 expression with immunomodulator and chemokine was predicted via the TISIDB database.Results: The expression of KRT19 was significantly upregulated in ovarian samples compared with normal controls. KRT19 expression was negatively associated with prognosis in OV, and further analysis revealed that KRT19 had promising diagnostic significance in distinguishing OV cancer from normal samples. GO-KEGG and GSEA analysis indicated that KRT19 was associated with multiple biological functions and pathways including epidermis development, apical junction, inflammatory response, and epithelial mesenchymal transition. By using different GEO series, we found that KRT19 was differentially expressed in OV-associated tissues. Furthermore, the increased KRT19 expression was positively correlated with the immune infiltration levels of the most immune cells in OV.Conclusion: This study demonstrated that KRT19 is a promising prognosis and diagnosis biomarker that determines cancer progression and is correlated with tumor immune cells infiltration in OV, suggesting being a molecular target for immunotherapies.Keywords: KRT19, OV cancer, prognosis, biomarker, immune infiltration
