Scientific Reports (Nov 2022)

Mass cytometry reveals cladribine-induced resets among innate lymphoid cells in multiple sclerosis

  • F. T. Aglas-Leitner,
  • P. Juillard,
  • A. Juillard,
  • S. N. Byrne,
  • S. Hawke,
  • G. E. Grau,
  • F. Marsh-Wakefield

DOI
https://doi.org/10.1038/s41598-022-24617-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 11

Abstract

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Abstract Here we present a comprehensive mass cytometry analysis of peripheral innate lymphoid cell (ILC) subsets in relapsing/remitting MS (RRMS) patients prior to and after onset of cladribine tablets (CladT). ILC analysis was conducted on CyTOF data from peripheral blood mononuclear cells (PBMC) of MS patients before, 2 and 6 months after onset of CladT, and non-MS controls. Dimensionality reduction was used for immunophenotyping ILC subsets. CladT reduced all ILC subsets, except for CD56bright NK cells and ILC2. Furthermore, CD38+ NK cell and CCR6+ ILC3 were excluded from CladT-induced immune cell reductions. Post-CladT replenishment by immature ILC was noted by increased CD5+ ILC1 proportions at 2 months, and boosted CD38−CD56bright NK cell numbers at 6 months. CladT induce immune cell depletion among ILC but exclude CD56bright NK cells and ILC2 subsets, as well as CD38+ NK cell and CCR6+ ILC3 immunophenotypes. Post-CladT ILC expansions indicate ILC reconstitution towards a more tolerant immune system phenotype.