Status epilepticus-induced changes in the subcellular distribution and activity of calcineurin in rat forebrain

Jonathan E Kurz; Annu Rana; J.Travis Parsons; Severn B Churn

doi:10.1016/j.nbd.2003.08.018

Neurobiology of Disease (Dec 2003)

Status epilepticus-induced changes in the subcellular distribution and activity of calcineurin in rat forebrain

Jonathan E Kurz,
Annu Rana,
J.Travis Parsons,
Severn B Churn

Affiliations

Jonathan E Kurz: Department of Neurology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Physiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA
Annu Rana: Department of Neurology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Physiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA
J.Travis Parsons: Department of Neurology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Physiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA
Severn B Churn: Department of Neurology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Physiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA; Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA

DOI: https://doi.org/10.1016/j.nbd.2003.08.018
Journal volume & issue: Vol. 14, no. 3
pp. 483 – 493

Abstract

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This study was performed to determine the effect of prolonged status epilepticus on the activity and subcellular location of a neuronally enriched, calcium-regulated enzyme, calcineurin. Brain fractions isolated from control animals and rats subjected to pilocarpine-induced status epilepticus were subjected to differential centrifugation. Specific subcellular fractions were tested for both calcineurin activity and enzyme content. Significant, status epilepticus-induced increases in calcineurin activity were found in homogenates, nuclear fractions, and crude synaptic membrane-enriched fractions isolated from both cortex and hippocampus. Additionally, significant increases in enzyme levels were observed in crude synaptic fractions as measured by Western analysis. Immunohistochemical studies revealed a status epilepticus-induced increase in calcineurin immunoreactivity in dendritic structures of pyramidal neurons of the hippocampus. The data demonstrate a status epilepticus-induced increase in calcineurin activity and concentration in the postsynaptic region of forebrain pyramidal neurons.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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