Detection of Alloxan induced Lipofuscin pigmentation in the cells of renal tubule of diabetic rats

Abstract

Objective: Diabetes mellitus is a metabolic disease due to failure in insulin secretion function. Kidney is a target organ in diabetes. Diabetic nephropathy is a major cause of end stage renal disease and resulting in increased morbidity and mortality of affected individuals. Since diabetes is a progressive disease, early diagnosis serves better treatment. Methods: We induced diabetes in rat by alloxan administration after 8 weeks. We detected lipofuscin products with H;E, PAS and schmorl’s staining methods. Findings: Diabetic rats showed increasing lipofuscin in renal proximal tubular cells. Conclusion: Increased lipofuscin pigments is a good indicator for diagnosis of diabetes in rats.

Keywords

• diabetes mellitus
• lipofuscin
• alloxan
• kidney