Development and Characterization of Biointeractive Gelatin Wound Dressing Based on Extract of <i>Punica granatum</i> Linn
Marismar F. do Nascimento,
Juliana C. Cardoso,
Tarsizio S. Santos,
Lívia A. Tavares,
Tatiana N. Pashirova,
Patricia Severino,
Eliana B. Souto,
Ricardo L. C. de Albuquerque-Junior
Affiliations
Marismar F. do Nascimento
Health and Environment Post-Graduating Program, University Tiradentes (UNIT), Aracaju 49032-490, Sergipe, Brazil
Juliana C. Cardoso
Health and Environment Post-Graduating Program, University Tiradentes (UNIT), Aracaju 49032-490, Sergipe, Brazil
Tarsizio S. Santos
Health and Environment Post-Graduating Program, University Tiradentes (UNIT), Aracaju 49032-490, Sergipe, Brazil
Lívia A. Tavares
Health and Environment Post-Graduating Program, University Tiradentes (UNIT), Aracaju 49032-490, Sergipe, Brazil
Tatiana N. Pashirova
Department of Pharmaceutical Technology, Faculty of Pharmacy (FFUC), University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
Patricia Severino
School of Pharmacy, Industrial Biotechnology Post-Graduating Program, University Tiradentes, Aracaju 49032-490, Sergipe, Brazil
Eliana B. Souto
Department of Pharmaceutical Technology, Faculty of Pharmacy (FFUC), University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
Ricardo L. C. de Albuquerque-Junior
Health and Environment Post-Graduating Program, University Tiradentes (UNIT), Aracaju 49032-490, Sergipe, Brazil
Punica granatum Linn (pomegranate) extracts have been proposed for wound healing due to their antimicrobial, antioxidant, and anti-inflammatory properties. In this work, we designed biointeractive membranes that contain standard extracts of P. granatum for the purpose of wound healing. The used standard extract contained 32.24 mg/g of gallic acid and 41.67 mg/g of ellagic acid, and it showed high antioxidant activity (the concentration of the extract that produces 50% scavenging (IC50) 1.715 µg/mL). Compared to the gelatin-based membranes (GEL), membranes containing P. granatum extracts (GELPG) presented a higher maximal tension (p = 0.021) and swelling index (p = 0.033) and lower water vapor permeability (p = 0.003). However, no difference was observed in the elongation and elastic modulus of the two types of membranes (p > 0.05). Our wound-healing assay showed that a GELPG-treated group experienced a significant increase compared to that of the control group in their wound contraction rates on days 3 (p p p P. granatum extracts has a promising potential application for dressings that are used to treat wounds.
