Separation and Identification of many Volatile oils from the Seeds and Peels of the Fruit of Iraqi Citrullus colocynthis (L.) Schrad Plant and Study its Antioxidant Effect

Abstract

A current study was included the separation and diagnosis of a number of volatile oils components from the seeds and the peels of a fruit from the Iraqi Citrullus colocynthis ( L. ) Schrad Plant , by using Clevenger apparatus for light compounds and the results were confirmed by using GLC technique .The volatile compounds were identified which included the following : (α-pinene, Limonene, Camphene, Carvacrol, Camphor, Pcymene, Terpinene, Linalool, Menthol, Sabinen and phellanodrene). Terpinene was the highest compound concentration in the seeds and peels %(12.14,16.58) respectively , whereas the lowest concentration in the seeds and peels was Menthol (0.08,0.14) % respectively. The study was also included studying the antioxidant effect of volatile oil separated from the seeds and peels at different concentrations and compared them with the ascorbic acid as a control sample, it was observed that the separated volatile oils from the peels were significantly superior of the free radical inhibition of DPPH (76.1,76.8) % at a concentration (300, 400) Mg.ml–1 respectively compared to the separated volatile oils from seeds.

Keywords

• Citrullus colocynthis (L.) Schrad , volatile oil , antioxidant , free radical , ascorbic acid , DPPH