Incidence of Vancomycin Resistant Staphylococci from Various Clinical Isolates In a Tertiary Care Hospital

Abstract

Vancomycin, a glycopeptide antibiotic, is the main antimicrobial agent available to treat life-threatening infections with Methicillin resistant Staphylococcus. 500 Staphylococcal isolates were speciated by conventional biochemical tests, as S.aureus and Coagulase negative Staphylococci (CONS). Isolates were screened for Vancomycin resistance by disc diffusion, inoculation on vancomycin screen agar & confirmed by E test. Vancomycin resistant isolates were tested for their susceptibility to Linezolid & Quinupristine-dalfopristine using the disc diffusion method. 294/500 isolates were S.aureus of which 155 were MRSA & 206/500 were CONS out of which 122 were MRCONS. Of the 3 vancomycin resistant isolates, one was VRSA and 2 were VRCONS, which were sensitive to linezolid, quinupristin/ dalfopristin.

Keywords

• s.aureus
• vrsa
• vrcons
• vancomycin screen agar