Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2)
Johannes Grosch,
Bernadette Eberlein,
Sebastian Waldherr,
Mariona Pascal,
Britta Dorn,
Clara San Bartolomé,
Federico De La Roca Pinzón,
Maximilian Schiener,
Ulf Darsow,
Tilo Biedermann,
Jonas Lidholm,
Maria Beatrice Bilò,
Thilo Jakob,
Carsten B. Schmidt-Weber,
Simon Blank
Affiliations
Johannes Grosch
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, Germany
Bernadette Eberlein
Department of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, Germany
Sebastian Waldherr
Department of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, Germany
Mariona Pascal
Immunology Department, CDB Hospital Clinic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, 08007 Barcelona, Spain
Britta Dorn
Experimental Dermatology and Allergy Research Group, Department of Dermatology and Allergology, Justus-Liebig-University Gießen, 35392 Gießen, Germany
Clara San Bartolomé
Immunology Department, CDB Hospital Clinic de Barcelona, Universitat de Barcelona, 08007 Barcelona, Spain
Federico De La Roca Pinzón
Allergy Section, Hospital Clinic de Barcelona, Clinical Medical Group, 08036 Barcelona, Spain
Maximilian Schiener
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, Germany
Ulf Darsow
Department of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, Germany
Tilo Biedermann
Department of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, Germany
Jonas Lidholm
Thermo Fisher Scientific, 75450 Uppsala, Sweden
Maria Beatrice Bilò
Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60121 Ancona, Italy
Thilo Jakob
Experimental Dermatology and Allergy Research Group, Department of Dermatology and Allergology, Justus-Liebig-University Gießen, 35392 Gießen, Germany
Carsten B. Schmidt-Weber
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, Germany
Simon Blank
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, Germany
Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (Apis mellifera) venom, HBV; Pol d 2 from European paper wasp (Polistes dominula) venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket (Vespula vulgaris) venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201.
