The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots

Abstract

The aim of the study was to test the influence of the mineral salt composition of the medium on in vitro multiplication and growth of kohleria shoots. Shoot tips were cultured on Murashige and Skoog (MS) (full- and half strength), Gamborg et al. (B5), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA 1 mg·dm-3. The influence of passage time on the induction and growth of shoots on the medium containing half strength of MS mineral salts and BA 1 mg·dm-3, GA3 1 mg·dm-3 was also studied. The explants used in the experiments were obtained from aseptically grown shoot clusters. A significant influence of the medium type and the time of propagation on the number, length and weight of axillary shoots was observed. The medium containing full or half strength of mineral salts according to MS was the best. The propagation of kohleria shoots should take place at 4-week passages.

Keywords

• Kohleria amabilis
• in vitro
• mineral salt composition
• time of passage