Nature Communications (Feb 2018)
3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection
Abstract
Long-term in vitro models for hepatitis B virus (HBV) infection are important to understand this infection, but are lacking. Here the authors develop a microfluidic primary human hepatocyte organoid culture system that can be maintained over 40 days and recapitulates all of the steps of the HBV life cycle.