International Journal of Nanomedicine (Nov 2022)
Multifunctional Mesoporous Silica Nanoparticles Reinforced Silk Fibroin Composite with Antibacterial and Osteogenic Effects for Infectious Bone Rehabilitation
Abstract
Dexiong Li,1,* Jing Xie,1,* Yubei Qiu,1,2 Sihui Zhang,1,3 Jiang Chen1,* 1School and Hospital of Stomatology, Fujian Medical University, Fuzhou, People’s Republic of China; 2Fujian Key Laboratory of Fujian College and University, Fujian Medical University, Fuzhou, People’s Republic of China; 3Institute of Stomatology & Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiang Chen, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, People’s Republic of China, Tel +86 591 83735488, Fax +86 591 83700838, Email [email protected]: Existing implant materials cannot meet the essential multifunctional requirements of repairing infected bone defects, such as antibacterial and osteogenesis abilities. A promising strategy to develop a versatile biomimicry composite of the natural bone structure may be accomplished by combining a multifunctional nanoparticle with an organic scaffold.Methods: In this study, a quaternary ammonium silane-modified mesoporous silica containing nano silver (Ag@QHMS) was successfully synthesized and further combined with silk fibroin (SF) to fabricate the multifunctional nano-reinforced scaffold (SF-Ag@QHMS) using the freeze-drying method. Furthermore, the antibacterial and osteogenic effects of this composite were evaluated in vitro and in vivo.Results: SF-Ag@QHMS inherited a three-dimensional porous structure (porosity rate: 91.90 ± 0.62%) and better mechanical characteristics (2.11 ± 0.06 kPa) than that of the SF scaffold (porosity rate: 91.62 ± 1.65%; mechanic strength: 2.02 ± 0.01 kPa). Simultaneously, the introduction of versatile nanoparticles has provided the composite with additional antibacterial ability against Porphyromonas gingivalis, which can be maintained for 15 days. Furthermore, the expression of osteogenic-associated factors was up-regulated due to the silver ions eluting from the composite scaffold. The in vivo micro-CT and histological results indicated that the new bone formation was not only localized around the border of the defect but also arose more in the center with the support of the composite.Conclusion: The multifunctional silver-loaded mesoporous silica enhanced the mechanical strength of the composite while also ensuring greater and sustained antibacterial and osteogenic properties, allowing the SF-Ag@QHMS composite to be used to repair infected bone defects.Keywords: silk fibroin, quaternary ammonium silane, multifunctional, nano-reinforce, composite scaffold
