Endogenous Intoxication and the Role of Antioxidants in Motion Activity Correction with Traumatic Brain Injury in Rat Model

Abstract

The aim of this study was to assess the effectiveness of cytoflavin and mexicor in the posttraumatic period of traumatic brain injury (TBI). Materials and Methods: The experiments were carried out on 60 white non pedigree female rats weighing 180-200 g, with 15 rats in each series. TBI was modeled by a free-falling weight drop of 100 g from a height of 80 cm on the parietal-occipital area of the head. Blood samples were taken from the sublingual vein in an amount of 2.0 ml on Days 1, 3, 7, and 12 after the alteration. After TBI, in Group 1, 15 rats received an intraperitoneal injection of 2-ethyl-6-methyl-3-hydroxypyridine succinate (mexicor) for 10 days in a daily dose of 8.0 mg/kg. In Group 2, 15 rats received an intraperitoneal injection of cytoflavin for 10 days in a daily dose of 0.2 ml/kg. The activity of lipid peroxidation (LPO) and antioxidant protection system (ASO) in the blood plasma was determined by a biochemicoluminescence method. Analysis of animal motion activity included the determination of the ability to balance and to stay at the wooden bar, time spent for moving on the bar from the bright light source to the darkroom, and paw slip frequency. Results: Mexicor and cytoflavin decreased the level of oxidative processes in rat model with TBI and the development of secondary brain injury. The positive dynamics in restoring pro- and antioxidant system balance was combined with positive changes in motor function.

Keywords

• traumatic brain injury
• rats
• lipid peroxidation
• antioxidant protection system