Biological Performances of Plasmonic Biohybrids Based on Phyto-Silver/Silver Chloride Nanoparticles
Yulia Gorshkova,
Marcela-Elisabeta Barbinta-Patrascu,
Gizo Bokuchava,
Nicoleta Badea,
Camelia Ungureanu,
Andrada Lazea-Stoyanova,
Mina Răileanu,
Mihaela Bacalum,
Vitaly Turchenko,
Alexander Zhigunov,
Ewa Juszyńska-Gałązka
Affiliations
Yulia Gorshkova
Joint Institute for Nuclear Research, Frank Laboratory of Neutron Physics, Joliot-Curie, 6, 141980 Dubna, Russia
Marcela-Elisabeta Barbinta-Patrascu
Department of Electricity, Solid-State Physics and Biophysics, Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG-11, 077125 Magurele, Romania
Gizo Bokuchava
Joint Institute for Nuclear Research, Frank Laboratory of Neutron Physics, Joliot-Curie, 6, 141980 Dubna, Russia
Nicoleta Badea
General Chemistry Department, Faculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1-7, Polizu Street, 011061 Bucharest, Romania
Camelia Ungureanu
General Chemistry Department, Faculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1-7, Polizu Street, 011061 Bucharest, Romania
Andrada Lazea-Stoyanova
National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania
Mina Răileanu
Department of Electricity, Solid-State Physics and Biophysics, Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG-11, 077125 Magurele, Romania
Mihaela Bacalum
Department of Life and Environmental Physics, Institute for Physics and Nuclear Engineering, Horia Hulubei National, Reactorului, 30, 077125 Magurele, Romania
Vitaly Turchenko
Joint Institute for Nuclear Research, Frank Laboratory of Neutron Physics, Joliot-Curie, 6, 141980 Dubna, Russia
Alexander Zhigunov
Institute of Macromolecular Chemistry AS CR, Heyrovskeho nam. 2, 162 06 Prague 6, Czech Republic
Ewa Juszyńska-Gałązka
Institute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Krakow, Poland
Silver/silver chloride nanoparticles (Ag/AgClNPs), with a mean size of 48.2 ± 9.5 nm and a zeta potential value of −31.1 ± 1.9 mV, obtained by the Green Chemistry approach from a mixture of nettle and grape extracts, were used as “building blocks” for the “green” development of plasmonic biohybrids containing biomimetic membranes and chitosan. The mechanism of biohybrid formation was elucidated by optical analyses (UV–vis absorption and emission fluorescence, FTIR, XRD, and SAXS) and microscopic techniques (AFM and SEM). The aforementioned novel materials showed a free radical scavenging capacity of 75% and excellent antimicrobial properties against Escherichia coli (IGZ = 45 mm) and Staphylococcus aureus (IGZ = 35 mm). The antiproliferative activity of biohybrids was highlighted by a therapeutic index value of 1.30 for HT-29 cancer cells and 1.77 for HepG2 cancer cells. At concentrations below 102.2 µM, these materials are not hemolytic, so they will not be harmful when found in the bloodstream. In conclusion, hybrid systems based on phyto-Ag/AgClNPs, artificial cell membranes, and chitosan can be considered potential adjuvants in liver and colorectal cancer treatment.
