Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host

Abstract

Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.