Comparative analysis of blood lymphocytes of patients with acute and chronic myeloid leukemia by surface and intracellular α1-acid glycoprotein and fibronectin

Abstract

The exposition of α1-acid glycoprotein and fibronectin on the surface and inside the lymphocytes of healthy donors and hematological patients with acute and chronic myeloid leukemia were studied. Localization of glycoproteins was determined by flow cytometry using antibodies conjugated to fluorescence label. Number of lymphocytes from α1-acid glycoprotein exposed on the surface under conditions of acute myeloid leukemia was 2.1 times higher than in patients with chronic myeloid leukemia. There is a significantly increasing of binding of antibodies to α1-acid glycoprotein both in cytosol and on the plasma membrane of lymphocytes in acute myeloid leukemia compared with the control group and patients with chronic myeloid leukemia. There is a significantly increasing of fibronectin exposition on the plasma membrane of lymphocytes and decreasing of fibronectin exponation in these cells in patients with chronic myeloid leukemia. The results revealed differences in distribution of the studied proteins inside and on plasma membrane of lymphocytes in patients with acute and chronic myeloid leukemia, which may represent changes in expression of these proteins depending on type of myeloleucosis.

Keywords

• fibronectin
• α1-acid glycoprotein
• lymphocytes
• acute myeloid leukemia
• chronic myeloid leukemia